"Quantitative Studies of Binding and Trafficking of Endogenous and Genetically-Altered Epidermal Growth Factor Receptors in Cultured Fibroblasts."

在过去的十年中，在确定肽生长因子及其细胞表面受体调控哺乳动物细胞增殖的分子成分方面取得了重大进展。由于这一规则是当前基于细胞培养技术的可靠和可重复设计中的许多问题的基础，因此将这一进展转化为有用的定量工程术语至关重要。我们提出了初步的研究来验证成纤维细胞系对表皮生长因子（EGF）的有丝分裂反应性与EGF受体的转运特性有关的假设。将进行各种动力学EGF结合和解离研究，以便在数学模型中估计各种贩运常数的值。这些研究旨在确定贩运参数的任何变化是否与扩散反应有关。如果是这样的话，沿着这条路线进行实质性的研究项目将会受到激励。

Significant progress has been made during the past decade on identifying the molecular components of the regulation of mammalian cell proliferation by peptide growth factors and their cell surface receptors. Since this regulation underlies many current problems in the reliable and reproducible design of cell culture-based technologies, a translation of this progress into useful quantitative engineering terms is crucial. Preliminary studies are proposed to test the hypothesis that the mitogenic responsiveness of fibroblast cell lines to epidermal growth factor (EGF) is related to the trafficking properties of the EGF receptor. A variety of kinetic EGF binding and dissociation studies will be performed in order to estimate values for various trafficking constants in a mathematical model. These studies intend to determine whether any changes in trafficking parameters are correlated to proliferation responses. If this is so, a substantial research program along these lines will be motivated.