The Kinetics of Growth and Product Release from Transformed Roots Using Nutrient Mists

植物是精细化学品的主要来源（次要来源） 代谢物），包括药物、香料、调味剂和 农药 尽管在这方面取得了有限的成功， 从植物培养物中生产有利可图的二次产品 人们对利用植物细胞和组织 这些有价值的商品的生产。 许多 这些产品都是由根产生的，所以使用植物 转化农杆菌，产生毛状根，提供了巨大的 商业开发潜力。 毛根是 基因稳定，生长迅速，并产生二级水平的 产品等于或大于整个工厂。 根毛， 然而，在传统的生物反应器中很容易被损坏。 一 这些植物组织用于商业用途的进一步障碍 规模是大多数次级代谢物积累在 植物组织中，并且不容易输出到生长培养基中。 因此，需要非破坏性方法来培养 毛状根和由这些毛状根生产的产品的方法 植物组织可以回收用于加工， 保留生物质。 拟议研究的目标是 在一个新的生物反应器中培育甜菜的毛状根 它使用营养雾，并测试物理技术， 用于释放产物的细胞的可逆透化 进入介质。 拟议的研究将为开发 一种独特的植物毛状体大规模生长模型系统 的根源和发展技术， 商业上有价值的产品（次级代谢产物） 专门的植物组织

Plants are a major source of fine chemicals (secondary metabolites) including pharmaceuticals, fragrances, flavors, and pesticides. Although there has been but limited success in the profitable production of secondary products from plant cultures there is serious interest in the use of plant cell and tissue culture for the production of these valuable commodities. Many of these products are produced by roots, so the use of plants transformed by Agrobacterium, yielding hairy roots, offers great potential for comercial exploitation. Hairy roots are genetically stable, grow rapidly, and produce levels of secondary products equal to or greater than the whole plant. Root hairs, however, are easily damaged in conventional bioreactors. A further barrier to the use of these plant tissues on a commercial scale is that most secondary metabolites accumulate within the plant tissue and are not readily exported into the growth medium. Thus, there is a need for nondestructive methods for culturing hairy roots and methods by which the products produced by these plant tissues can be recovered for processing while still retaining the biomass. The goals of the proposed research are to grow hairy roots of beets (Beta vulgaris) in a new bioreactor which employs nutrient mists, and to test physical techniques for the reversible permeabilization of cells for release of products into the medium. The proposed research will lay the groundwork for development of a unique model system for the large scale growth of plant hairy roots and development of techniques for the recovery of commercially valuable products (secondary metabolites) from these specialized plant tissues.