Conformational Dynamics of Components of Protein Biosynthesis Systems

本研究的目的是调查 蛋白质生物合成组分构象动力学 细菌和高等生物的系统。 具体地说， 延伸因子从C.大肠杆菌和小牛脑中的蛋白质 研究了分离形式和与 从它们各自的蛋白质中提取合适的成分 生物合成系统。 在E.大肠杆菌蛋白 复合物将是鸟嘌呤核苷酸结合形式， 与另一种延伸因子形成的异二聚体， 与鸟嘌呤核苷酸形成三元复合物， 氨酰化-tRNA和与抗生素形成的复合物 金液。 小牛脑的延伸因子也将是 研究了它与鸟嘌呤核苷酸和 其他延伸因素。 这些研究的目标是 检查构象的性质和程度 在形成这些不同的变化时发生的变化 生物学上重要的复合物，以及阐明 复合物的流体动力学方面。 除了 提供这些重要的蛋白质成分的信息 生物合成系统，拟议的研究也将有 与其他奎宁核苷酸相关研究的相关性 结合蛋白

The object of this study is the investigation of the conformational dynamics of components of protein biosynthesis systems from bacterial and higher organisms. Specifically, elongation factors from c. coli and from calf brain will be studied both in isolated forms and in complexes with appropriate components from their respective protein biosynthesis systems. In the case of the E. coli protein, the complexes will be the guanine nucleotide bound forms, the heterodimer formed with still another elongation factor, the ternary complex formed with guanine nucleotide and aminoacylated-tRNA and the complex formed with the antibiotic aurodox. The elongation factor from calf brain will also be studied in its complexes with guanine nucleotides and with other elongation factors. The goal of these studies will be to examine the nature and extent of the conformational alterations which occur upon formation of these various biologically important complexes as well as to elucidate hydrodynamic aspects of the complexes. In addition to providing information on these important components of protein biosynthesis systems the proposed studies will also have relevance to related research on other quinine nucleotide binding proteins.