An Electronic Area Detector for Protein Crystallography

该提案要求提供资金，用于购买电子 X射线晶体学用面探测器衍射仪 研究蛋白质结构。 这是一个更新版本的 最初于1986年12月9日提交。 目前正在研究的蛋白质包括各种 E.大肠杆菌、鸡和人的二氢叶酸 还原酶、酵母细胞色素c的突变体和复合物 过氧化物酶。 此外，与 DNA复制和修复已经结晶， 研究以确定其结构的适用性 测定：E.大肠杆菌质粒和大鼠B-聚合酶。 还有一些蛋白质正在进行结晶试验。 获取区域检测器衍射仪将允许PI 在晶体准备好安装时收集数据， 无需等待，往往是几个月，以获得一个 衍射仪在另一个实验室;提高速率和 数据收集的灵活性;允许完整的数据集， 从最少数量的晶体中收集;并提供准确的 用于结构细化的高分辨率数据。

This proposal requests funds for the purchase of an electronic area detector diffractometer for use in x-ray crystallographic studies of protein structures. It is an updated version of the proposal originally submitted Dec. 9, 1986. Proteins currently being investigated include a variety of complexes and mutants of E. coli, chicken and human dihydrofolate reductase, and mutants and complexes of yeast cytochrome c peroxidase. Additionally, crystals of two proteins relevant to DNA replication and repair have been crystallized and are being studied to establish their suitability for structure determination: repA1 of an E. coli plasmid and rat B-ploymerase. Still other proteins are undergoing crystallization trials. Acquisition of an area detector diffractometer will allow the PIs to collect data when crystals are ready for mounting and eliminate the need to wait, often several months, for access to a diffractometer in another laboratory; enhance the rate and flexibility of data collection; permit complete data sets to be collected from a minimum number of crystals; and provide accurate high-resolution data for structure refinement.