Clathrate Hydrates in Protein-Containing Reversed Micellar System

本研究探讨了笼形物的影响， 反胶束溶液中水合物的形成 蛋白质的溶解性和构象。 初步 结果表明，水合物的形成，在一定的 影响蛋白质溶解成胶束的条件 当传统的相转移方法证明 无效。 还发现水合物 形成是一种原位修饰胶束的方法 蛋白质构象和酶的大小 活动 因此，提出了一系列相关研究 利用这些概念来提高蛋白质提取 转化为反胶束，并以适当的形式回收。 使用水合物来改变催化剂的催化活性， 还提出了反胶束中的酶。 的 反胶束中水合物形成的现象， 到目前为止基本上没有研究过。 在很大 从某种程度上说，这项研究是现象学的， 方面是探索性的。

This research investigates the effects of clathrate hydrate formation in reversed micellar solutions on the solubility and conformation of proteins. Preliminary results show that hydrate formation can, under certain conditions, effect protein solubilization into micelles when conventional phase transfer methods prove ineffective. It has also been found that hydrate formation is a method of in-situ modification of micelle size, and thereby, of protein conformation and enzyme activity. Therefore a set of related studies is proposed that exploit these concepts to enhance protein extraction into reversed micelles, and recovery in appropriate form. The use of hydrates to alter the catalytic activity of enzymes in reversed micelles is also proposed. The phenomenon of hydrate formation in reversed micelles is essentially unstudied up to the present. To a large extent, this research is phenomenological, and certain aspects are exploratory.