Cell Cycle Kinetic Phenomena In Monoclonal Antibody Production

鉴于单克隆抗体是较重要的具有商业价值的生化物质之一，本研究的目的是了解抗体合成的基本生物学现象，并将研究结果应用于生物反应器操作策略的改进。该项目专注于单克隆抗体生产可能的细胞周期依赖性，并将利用带有溴脱氧尿苷标记的流式细胞术来测量细胞周期参数，并利用分子生物学技术来探测抗体合成的转录和转录后调控。数据将被纳入使用细胞周期依赖动力学的数学模型中。该结果应提供实验数据的机制解释，并应导致更好地理解和改进单克隆抗体的生产。

With the view that monoclonal antibodies are among the more important biochemicals of commercial value, the objective of the proposed research is to understand the basic biological phenomena of antibody synthesis, and to apply the results to the improvement of bioreactor operating strategies. This project is focused on the probable cell cycle dependency of monoclonal antibody production, and will utilize flow cytometry with bromodeoxyuridine labeling to measure cell cycle parameters, and molecular biology techniques to probe transcriptional and post transcriptional regulation of antibody synthesis. Data are to be incorporated into mathematical models which use cell cycle dependent kinetics. The results should provide a mechanistic explanation of the experimental data, and should lead to a better understanding and improved production of monoclonal antibodies.