Multiple Frequency EPR/ENDOR Spectrometer System

要求提供经费，用于最先进的多频ESP-300E电子顺磁共振光谱仪系统，具有X波段能力的液氦和氮气可变温度系统、可变温度测角仪和微波计数器。这些研究设备将用于几个不同的项目。这些研究包括：模型血红素络合物、双组氨酸配位细胞色素b和c以及混合金属钴取代的血红蛋白的多频EPR研究；新分离的c型细胞色素和定点突变产生的突变细胞色素c的EPR研究；亚硫酸盐氧化酶和模型钼(V)配合物的钼和血红素中心的多频EPR研究；有机硫化合物的光化学或电化学产生的自由基的EPR研究和有机硫化合物的铜配合物的研究；EPR研究模型膜结合的多肽或蛋白质残基由于模型膜中脂类自旋标记的存在而导致的本征荧光猝灭，以及利用EPR研究模型膜组分的聚合动力学；以及EPR研究涉及蛋白质-小分子和蛋白质-蛋白质体系的电子转移过程的动力学。

Funding is requested for a state-of-the-art multiple frequency ESP-300E EPR spectrometer system with X-band capability liquid helium and nitrogen variable temperature systems, a variable temperature goniometer and a microwave counters. This research equipment will be used for several diverse projects. These include: multiple frequency EPR investigations of model heme complexes, bis-histidine coordinated cytochromes b and c, and mixed metal cobalt-substituted hemoglobins; EPR investigations of newly isolated c-type cytochromes and of mutant cytochromes c produced by site-directed mutagenesis; multiple frequency EPR investigations of the molybdenum and heme centers of sulfite oxidase and model molybdenum (V) complexes; EPR investigations of photochemically or electrochemically produced radicals of organosulfur compounds and investigations of copper complexes of organosulfur compounds; EPR studies of the quenching of the intrinsic fluorescence of model membrane-bound peptide or protein residue due to the presence of spin labels on lipids in the model membranes, and the use of EPR to investigate the kinetics of polymerization of model membrane component; and EPR investigation of the kinetics of electron transfer processes involving protein- small molecular and protein-protein systems.