Acquisition of 300MHz NMR Spectrometer

该提案要求为垂直钻孔提供部分资金， 高分辨中场核磁共振谱仪 结构生物学中的光谱学 该工具将包括 7.05 T磁体，10 mm的足够均匀体积 质子和20 mm异核样品管，以及一个控制台， 先进技术所需的所有功能，如质子 异核探测，自旋锁定，多量子 实验和所有其他现代二维方法。 光谱仪将由五个主要的核心小组使用 用户（四个由NSF资助）和几个小用户。 的 主要用户的项目侧重于NMR应用， 结构生物学 计划使用拟议的 光谱仪强调生物分子结构的研究， 蛋白质活性位点的模型化合物，主要在系统上 含有顺磁性金属中心。 每个调查员都有一个 建立了核磁共振研究的程序， 能力，建议书记录了额外的知识 将通过拟议仪器的新功能获得。 拟议的文书将由统一委员会主持 戴维斯核磁共振设备。 该设施有超过10个 多年来，在实施国家的最先进的核磁共振技术，并在 光谱仪维护和改造。

This Proposal requests partial funding for a vertical bore, intermediate field NMR spectrometer for high resolution spectroscopy in structural biology. The instrument will consist of a 7.05 T magnet, sufficient homogeneous volume for 10 mm proton and 20 mm heteronuclei sample tubes, and a console with all features necessary for advanced techniques such as proton detection of heteronuclei, spin locking, multiple quantum experiments, and all other modern two dimensional methods. The spectrometer will be utilized by a core group of five Major User (four with NSF funding) and several minor users. The projects of the Major Users focus on NMR applications to structural biology. Planned research using the proposed spectrometer emphasizes studies of biomolecule structure and model compounds of protein active sites, primarily on systems containing paramagnetic metal centers. Each investigator has an established program of NMR research with spectrometers of lesser capability, and the Proposal documents the additional knowledge to be gained by the new capabilities of the proposed instrument. The proposed instrument will come under the auspices of the UC Davis NMR Facility. The Facility has a proven record of over ten years in implementing state-of-the-art NMR techniques, and in spectrometer maintenance and modification.