ABR: Experiments in Deep-Sea Microbiology

这项研究将解决这样一个问题：在深海中负责矿化过程的微生物的生理特征是什么？更具体地说：深海细菌对温度和压力的适应是否随营养而不变？将使用已知的嗜水（耐压）生物的菌株，以及新鲜获得的深海分离株。将进行生长试验：1。作为常压、原位底物浓度和2-20℃温度范围下的连续培养实验；和2。在原位压力和2- 20℃的温度下分批培养，并在实验中尽可能低的浓度下培养营养基质。在实验条件下，种群密度（细胞计数和生物量）和/或放射性底物周转率的变化将被用作生长速度对各种可控环境因素（温度、压力和底物浓度）变化响应的敏感指标。这项研究可能会为深海环境中调节微生物生长的因素和微生物转化物质的速度提供新的见解。

This study will address the question: What are the physiological characteristics of those microorganisms that are responsible for the mineralization processes in the deep sea? And more specifically: Are the temperature and pressure adaptations of deep-sea bacteria invariable with nutrition? Strains from a stock of known barophilic (pressure tolerant) organisms, as well as freshly-obtained deep sea isolates, will be used. Growth experiments will be carried out: 1. as continuous culture experiments at atmospheric pressure, and at in situ substrate concentrations and a temperature range of 2-20 oC; and 2. as batch cultures at in situ pressures, and temperatures again of 2- 20 oC and nutrient substrates at the experimentally lowest possible concentrations attainable. Under experimental conditions, changes in population density (cell counts and biomass) and/or radioactive substrate turnover will be used as a sensitive indicator of growth rate response to changes of the various controllable environmental factors: temperature, pressure and substrate concentration. This study potentially will provide new insights into factors regulating microbial growth and the rates of microbial transformations of matter in deep sea environments.