Genetic Engineering of Plants with Antifreeze Proteins to Enhance the Quality of Frozen Plant Products

这项研究的目的是通过表达抑制冰晶生长的抗冻蛋白来培育具有增强抗冻性的转化植物品种。我们已经证明，转化了冬比目鱼抗冻基因的烟草和番茄植株表达了相应的高水平的mRNA。此外，对基因进行了修改，使编码的蛋白质更大，更容易被检测到。在含有该融合蛋白的植物中，抗冻蛋白在RNA、蛋白质和活性水平上都有表达，其中转录水平占主导地位。我们现在将评估抗冻蛋白对转基因植物果实以及渗透了抗冻蛋白的非转基因水果耐寒性的影响。如果有必要，我们将通过提高蛋白质活性、蛋白质稳定性、转录和可译性来优化植物组织中的蛋白质浓度。产生最佳耐寒性的建筑将被转移到适当的商业品种。*/

The aim of the research described is to develop transformed plant varieties with enhanced freezing tolerance through the expression of antifreeze proteins that inhibit ice crystal growth. We have shown that tobacco and tomato plants transformed with a winter flounder antifreeze gene express high levels of the corresponding mRNA. Furthermore, the gene was modified so that the encoded protein is larger and more readily detected. In plants containing this fusion protein, antifreeze expression was observed at the RNA, protein and activity level, with transcriptional levels predominating. We will now evaluate the impact of antifreeze protein on the freezing tolerance properties of fruit from transgenic plants as well as from nontransgenic fruits that have been infiltrated with antifreeze protein. If necessary, we will optimize the protein concentration in plant tissue by enhancing protein activity, protein stability, transcription, and translatability. Constructions resulting in optimal freezing tolerance will be transferred into appropriate commercial varieties.***//