Single Ion Channels From Teleost Osmoregulatory Tissues

脊椎动物的肠和膀胱 重要的屏障功能，控制营养物质的运动， 盐和水进出生物体。 主要目的 拟议项目的目的是确定具体机制， 这些物质通过这些组织和途径 控制这些过程。 单个膜蛋白 具体来说，可以研究 在它们的整体行为中使用传统的电生理学 技术（以及与整体宏观相关的结果） 组织在动物体内平衡中的功能）或 分别使用膜片钳技术进行研究（结果 涉及膜离子通道功能的生物物理学）。 这些 技术将被应用于描绘激素的作用， 改变膜转运的药物， 这些组织在盐和水平衡中的作用。 //

The intestine and urinary bladder of vertebrate animals serve an important barrier function controlling the movement of nutrients, salts and water into and out of the organism. The major objective of the proposed project is to determine the specific mechanisms by which such substances pass across these tissues and the pathways for control of these processes. The individual membrane proteins which mediate the movement of ions, specifically, can be studied in their ensemble behavior using conventional electrophysiological techniques (and the results related to the overall macroscopoic functioning of the tissue in homeostatic balance of the animal) or studied individually using patch clamp technique (and the results related to the biophysics of membrane ion channel function). These techniques will be applied to delineate the roles of hormones and drugs in altering membrane transport and to increase understanding of the role(s) of these tissues in salt and water balance. //