An Investigation of the Cellular Events that Control the Deposition of Patterned Walls in Plant Cells

本研究将探索分离的叶肉细胞同步分化形成管状细胞的独特发育系统。气管元件的特征是具有明显图案的次级壁，但尚不清楚壁沉积和图案是如何控制的。先前的研究结果表明，在细胞壁沉积之前和过程中需要钙的固存和运输，从而为细胞壁沉积中可能协调各种事件的调节离子提供了第一个证据。这些和其他结果将在三个方向上扩展：(1)确定壁沉积中的哪些事件是由钙调节的；(2)确定如何控制墙体图案，特别是关于墙体组件的自决定相互作用；(3)与细胞壁图案相关的质膜相关蛋白的表征。该研究将使用现代细胞化学、光谱学和分子生物学技术进行细胞壁结构的分子解剖和模式控制。细胞壁管状元件是植物发育生物学中的经典样本，在植物适应中起着重要作用，这将是首次尝试从分子角度了解细胞壁管状元件合成的时空控制。

This research will exploit a unique developmental system in which isolated mesophyll cells differentiate synchronously to form tracheary elements. Tracheary elements are characterized by distinctly patterned secondary walls, but it is not known how wall deposition and patterning are controlled. Prior results demonstrated requirements for calcium sequestration and transport before and during wall deposition, thereby providing the first evidence for a regulatory ion the might coordinate diverse events in cell wall deposition. These and other results will be extended in three directions: (1) Determination of which events in wall deposition are regulated by calcium; (2) Determination of how wall patterning is controlled, particularly in regard to self-determining interactions of wall components; and (3) Characterization of a plasma membrane associated protein associated with cell wall patterning. The research will use modern cytochemical, spectroscopic, and molecular biological techniques for molecular dissection of cell wall structure and control of patterning. It will represent the first attempt to obtain a molecular understanding of the temporal and spatial control of synthesis of cell wall tracheary elements, which are classical specimens in plant developmental biology and have a significant role in plant adaptation.