SCINTILLATION PROXIMITY FLUOROGRAPHY: A NEW METHOD FOR TRACKING BIOMOLECULES IN CELLS

该奖项的目的是测试使用的可行性 与生物素化抗体偶联的放射性 大分子前体和常规电子显微镜 放射自显影检测新合成的 大分子 这种方法，如果开发出来，将使研究人员 来追踪特定分子的动力学， 合成到细胞内的目的地。 可行性 试验内容包括：（1）rubisco在 浮萍叶绿体中过氧化氢酶的定位 过氧化物酶体;（3）液泡ATP酶的定位和追踪 在植物根尖中。

The objective of this award is to test the feasibility of the use of scintillant coupled to biotinylated antibodies, radioactive macromolecular precursors and conventional electron microscope autoradiography for the detection of newly-synthesized macromolecules. The method, if developed, will allow researchers to follow the dynamics of specific molecules from their site of synthesis to their destination within the cell. The feasibility test will be carried out on: (1) localization of rubisco in chloroplasts of Lemna, (2) localization of catalase in peroxisomes, and (3) localization and tracking of vacuolar ATPase in plant root tips.