Probing Ribosome - mRNA Interactions

希尔博士建议研究信使RNA(信使RNA)与核糖体结合时的结构。虽然在理解转移RNA(TRNA)与核糖体相互作用的细节方面已经取得了重大进展，但mRNA与核糖体的相互作用仍然不清楚。然而，要了解mRNA如何与核糖体、tRNA和其他参与翻译的因素相互作用是至关重要的。在这项提案中，希尔博士概述了使用短的、互补的DNA寡聚体来研究这种相互作用的方法。结果应该表明，当与核糖体结合时，信使核糖核酸的结构发生了变化。MRNAs通过核糖体的路径将被绘制出来。此外，还概述了一个合作项目，该项目使用短DNA低聚物在核糖体晶体中放置十碳金团簇。

Dr. Hill proposes to investigate the structure of messenger RNA (mRNA) while it is bound to the ribosome. Although significant progress has been made in understanding the details of transfer RNA (tRNA) interaction with the ribosome, the interaction of mRNA with the ribosome remains obscure. Yet, to understand how mRNA interacts with the ribosome, tRNA and other factors involved in translation is critical. In this proposal, Dr. Hill outlines methods by which this interaction can be studies using short, complementary DNA oligomers. The results should indicate where structural changes occur in the mRNA upon binding to the ribosome. The path of the mRNA through the ribosome will be mapped. In addition, a collaborative project in which short DNA oligomers are used to place undeca-gold clusters in ribosome crystals is outlined.//