U.S.-Thailand Cooperative Research in Renal Function in Vertebrates

这一建议要求资金允许威廉H。丹茨勒， 亚利桑那大学生理学系， Varanuj Chatsudthipong博士，生理学系，Mahidol 泰国国立大学，为期24个月， 肾小管转运有机阴离子。 这些研究将 涉及继续和扩大关于有机阴离子工作 基底膜和腔膜的转运步骤 哺乳动物肾小管 他们将使用隔离灌注和 在体外未灌注的肾小管中，在完整的肾小管中， 该模型基于来自膜囊泡的数据， 阴离子被输送到细胞中， 梯度离心 他们将利用类似的技术来评估 对氨基马尿酸盐跨腔膜转运 肾小管和评估可能的阴离子交换和参与 无机离子在这个运输过程中。 这些研究对于理解 肾功能，并在比较肾功能， 爬行动物和哺乳动物。 该项目增加了一个国际 合作方面的PI的研究下，美国国家科学基金会资助号。 DCB-9001985。 合作者都是德高望重的科学家 他们有成功的合作研究记录。 这 项目与科学的目标有关， 发展中国家计划，旨在增加 美国与发展中国家的合作水平 科学家和工程师通过科学交流， 信息、想法、技能和技术， 在互惠互利的问题上进行合作。

This proposal requests funds to permit Dr. William H. Dantzler, Department of Physiology, University of Arizona, to pursue with Dr. Varanuj Chatsudthipong, Department of Physiology, Mahidol University, Thailand, for a period of 24 months, research on the renal tubular transport of organic anions. The studies will involve a continuation and expansion of work on organic anion transport steps at both the basolateral and luminal membranes of mammalian renal tubules. They will utilize isolated perfused and nonperfused renal tubules in vitro to test, in intact tubules, the model, based on data from membrane vesicles, that organic anions are transported into cells against an electrochemical gradient. They will utilize similar techniques to evaluate p-aminohippurate transport across the luminal membrane in intact tubules and evaluate possible anion exchange and the involvement of inorganic ions in this transport process. These studies are important in gaining an understanding of how kidneys function and in making a comparison of kidney function in reptiles and in mammals. This project adds an international cooperative dimension to the PI's research under NSF Grant No. DCB-9001985. The collaborators are highly respected scientists who have a record of successful prior cooperative research. This project is relevant to the objectives of the Science in Developing Countries Program which seeks to increase the level of cooperation between U.S. and developing country scientists and engineers through the exchange of scientific information, ideas, skills, and techniques and through collaboration on problems of mutual benefit.