Acquisition of a Differential Scanning Calorimeter

我们要求差示扫描量热仪(DSC)配备扩展温度范围(220摄氏度)、外部冷却装置、数据收集和分析系统以及软件和可重复使用的电池。这是唯一具有所需灵敏度和温度范围的DSC。这台仪器的获得将使P.I.S能够分析嗜热和超高温生物体的酶的热变性特征，研究已知结构的小蛋白热变性的分子机制，分析具有生理意义的蛋白水解酶抑制剂作用的热力学基础，探讨亚基和结构域相互作用在酶活性产生中的意义。这些研究将扩大我们对蛋白质热稳定性和酶活性的结构基础的了解。

We are requesting for a differential scanning calorimeter ( DSC) equipped with extended temperature range (220C), an external cooling unit, data collection and analysis system and software and reusable cells. This is the only DSC with the requisite sensitivity and temperature range offered. The acquisition of this instrument will permit the P.I.'s to analyze the thermal denaturation characteristics of enzymes from thermophilic and superthermophillic organisms, to study the molecular mechanism of thermal denaturation of small proteins of know structure, to analyze thermodynamic basis of action of a protease inhibitor of physiological significance and to explore the significance of subunit and domain interactions in producing activity in an enzyme. These studies will extend our knowledge of the structural basis of thermal stability in proteins and activity in enzymes.