New Directions for Enzyme-Linked Binding Assays

这个项目是在分析和表面化学的一般领域和生物分析的子领域。在这项为期三年的持续资助期间，Meyerhoff教授和他的学生将在几种新的生物分子结合分析方法中使用酶作为标签进行基础研究。利用酶联结合测定法（ELBAs）的三个具体研究重点将被追求，即：(1)开发和研究一种新的基于凝集素的均质ELBA；为了合成更多有用的酶-寡糖偶联物，并证明这种偶联物/凝集素系统可以用于快速探测完整糖蛋白的碳水化合物结构；(2)评估使用多种酶标签结合多种结合剂对单一混合物中生物分子同时进行ELBAs的适用性和分析局限性；(3)探索原位电化学释放底物的可行性，用于ELBAs中检测特定蛋白质的潜在用途。这项研究建立在美国国家科学基金资助CHE-的基础上

This project is in the general area of analytical and surface chemistry and in the subfield of bioanalysis. During the tenure of this three-year continuing grant, Professor Meyerhoff and his students will pursue fundamental studies relating to the use of enzymes as labels in several novel binding assay methods of analysis of biomolecules. Three specific research thrusts utilizing enzyme-linked binding assays (ELBAs) will be pursued, namely (1) the development and study of a new lectin-based homogeneous ELBA, with the specific intent of synthesizing more useful enzyme-oligosaccharide conjugates and demonstrating that such conjugate/lectin systems can be used to probe rapidly the carbohydrate structures of intact glycoproteins; (2) assessment of the applicability and analytical limits of using multiple enzyme labels in conjunction with multiple binders to perform simultaneous ELBAs of biolmolecules in a single mixture; and (3) exploration of the feasibility of implementing in situ electrochemical release of substrates for potential use in ELBAs for the detection of specific proteins. %%% This research builds upon results obtained under NSF grant CHE-