Instrumentation for the Expansion of the Advanced Recombinant DNA Techniques Laboratory

上述课程的目的是向学生介绍有关使用和合成寡核苷酸作为诱变探针、引物和工具的最新程序和仪器。该仪器包括DNA合成器、高压液相色谱系统和DNA时间周期仪，用于合成寡核苷酸、利用HPLC分离寡核苷酸、质粒和pcr生成的DNA片段，以及利用寡核苷酸引入突变。

The purpose of the above course is to introduce students to state-of-the-art procedures and instrumentation relating to the use and synthesis of oligonucleotides as probes, primers and instruments of mutagenesis. The instrumentation, which includes a DNA synthesizers, high pressure liquid chromatographic systems and DNA tempcyclers, is used in the synthesis of oligonucleotides, the isolation of oligonucleotides, plasmids and PCR-generated DNA fragments using HPLC, and to introduce mutations using oligonucleotides.