Cell Biology Laboratory For Advanced Undergraduates

这个项目的目标是使用组织培养技术向高水平的生物学学生介绍分子细胞生物学的最新概念。购买了无菌罩二氧化碳培养箱、倒置和荧光显微镜、冷藏保温瓶和垂直平板凝胶电泳系统来实施这些实验。学生使用超速离心法从培养细胞中分离出膜组分。膜和整个细胞使用针对特定蛋白质和细胞器的一次抗体以及过氧化物酶标记和荧光标记的二次抗体进行染色。使用原代鸡成纤维细胞和劳斯肉瘤病毒演示病毒转化，学生使用倒置显微镜和脉冲标记技术观察和量化转化。化学信号是通过向BC3H1细胞系添加特定的激素来证明的，这些激素导致这些细胞分化为肌肉表型。Western和Northern印迹也在组织培养细胞中得到证实。

The goal of this project is to use tissue culture techniques to introduce upper-level biology students to current concepts in molecular cell biology. A sterile hood CO2 incubator, inverted and epifluoresence microscopes, a cryo-storage thermos, and a vertical slab gel electrophoresis system were purchased to implement these experiments. Students isolate membrane fractions from cultured cells using ultracentrifugation techniques. Membranes and whole cells are stained using primary antibodies to specific proteins and organelles and peroxidase-labelled and fluorescent-labelled secondary antibodies. Viral transformation is demonstrated using primary chick fibroblasts and Rous sarcoma virus, and students observe and quantitate the transformation utilizing the inverted microscope and pulse-labelling techniques. Chemical signaling is demonstrated through the addition of defined hormones to a BC3H1 cell line that cause differentiation of these cells into the muscle phenotype. Western and Northern blotting are also demonstrated with tissue-cultured cells.