An Adhesion-deficient Methylosinus Trichosporium OB3b for In Situ Bioremediation of Trichloroethylene-contaminated Aquifers

污染控制领域需要一种高效、成本低的- 补救含水层的有效就地处理程序 被氯化脂肪族化合物污染， 三氯乙烯（TCE）。 成功地在现场处理饱和的 区域土壤和液体需要存在代谢 在污染的目标区域的能力的细菌。 在许多情况下， 代谢所需的微生物可能不存在于 污染区，或可能不均匀地分布在整个 地下。 三氯乙烯是一种常见的地下水污染物， 被好氧甲烷氧化菌Methylosinus高效降解 发孢霉OB 3b. 然而，M.像大多数土壤一样， 隔离物，具有天然的粘合性能， 土壤表面，从而限制了它们的可及性， 污染物 我们正在制造一种粘附缺陷突变体， OB 3b能够通过含水层移动，同时积极降解 TCE及相关有毒物质。具有这种能力的有机体 将作为具有成本效益的原位处理的基础 技术，或在与 目前净化含水层的泵和处理方法。

The pollution control field is in need of an efficient, cost- effective in situ treatment process to remediate aquifers contaminated with chlorinated aliphatic compounds such as trichloroethylene (TCE). Successful in situ treatment of saturated zone soils and liquid requires the presence of metabolically capable bacteria in the polluted target zone. In many cases, metabolically desirable microorganisms may not be present in the contaminated zone, or may be unevenly distributed throughout the subsurface. TCE is a common ground water contaminant that is very efficiently degraded by the aerobic methanotroph Methylosinus trichosporium OB3b. However, M. trichosporium, like most soil isolates, has natural adhesive properties which cause them to bind to soil surfaces, thus limiting their accessibility to the contaminant. We are generating an adhesion deficient mutant of OB3b capable of moving through an aquifer while actively degrading TCE and related toxic substances. An organism with this capability would serve as the basis for a cost-effective in situ treatment technology, or provide synergistic benefits when combined with current pump-and-treat methods of purifying aquifers.