Molecular Genetics of Arabidopsis Thaliana

该提案有四个独立的实验部分， 他们的目标是试图解决 一组调控基因的活动如何 未分化的花原基形成复杂而精确的阵列 许多分化的细胞类型。 提出的实验是1） 研究两个花的调控基因的分子细节， 我们已经克隆了（APETALA 3和PISTILLATA）; 2）克隆和 类似地研究地籍基因SUPERMAN 3）以发现新的基因 对花的发育有调节作用， 与调控基因相互作用，其研究应该揭示， 关于花卉图案的形成， 迄今已知的同源异型和地籍基因的研究;和4） 将野生型和突变型花发育的描述， 一个新的水平的细胞分析，使用激光扫描共聚焦 microsopy，作为回答许多遗留问题的一种方式 迄今为止所做的分析没有回答这个问题。 %%% 植物开花，然后生产，因此，虽然拟南芥是杂草 而且没有商业上的顾虑，如果了解花是如何 发展是遗传控制的结果，从这项工作， 经济影响重大。

This proposal has four separate experimental parts, all related by their goal of attempting to solve different aspects of the problem of how a set of regulatory gene activities turns undifferentiated floral primordia into complex and precise arrays of many differentiated cell types. The experiments proposed are 1) to study in great molecular detail two floral regulatory genes that we have already cloned (APETALA3 and PISTILLATA); 2) to clone and similarly study the cadastral gene SUPERMAN 3) to find new genes with regulatory effects on flower development, and others which interact with the regulatory genes, whose study should reveal as much about floral pattern formation as has been revealed by the study of the homeotic and cadastral genes so far known; and 4) to take the description of wild-type and mutant flower development to a new level of cellular analysis, using laser scanning confocal microsopy, as a way of answering many of the questions left unanswered by the analyses that have been done to date. %%% Plants flower and then produce, thus although Arabidopsis is a weed and of no commercial concern, if an understanding of how flower development is genetically controlled results from this work, the economic implications are significant.