Temporal and Spatial Regulation of the Putative Class II H+ Transporter of the Chara Plasma Membrane

该项目的重点是监管级联， 最终激活了巨星系中的离子传输 节间细胞。在光照下，离子 这些细胞中的转运活动在空间上迅速变得 分离成交替的质子带 流入和流出。实验旨在辨别 蛋白质去磷酸化在激活 离子的快速空间分离 运动活动。 %%% 离子穿过植物细胞质膜的运动 受到严格的监管控制，并对各种 细胞外信号。轮藻属的巨大节间细胞 Corpera为这些研究提供了独特的模型系统， 调节级联，因为离子运动是由光触发的， 似乎与光合作用直接相关。的 信号转导的中间步骤还不清楚 也是这个项目的焦点之一的转导 这些信号可以为了解机制提供重要线索， 植物细胞调节质膜上的运输。

This project is focused on the regulatory cascade that culminates in the activation of ion transport in the giant internodal cell of Chara corallina. Upon illumination, ion transport activity in these cells rapidly becomes spatially segregated into alternating bands of what appears to be proton influx and efflux. Experiments are aimed at discerning the potential role of protein dephosphorylation in the activation of the transport system and in the rapid spatial segregation of ion movement activities. %%% The movement of ions across the plasma membrane of plant cells is under strict regulatory control and is responsive to a variety of extracellular signals. The giant internodal cell from Chara corallina provides a unique model system for the study of these regulatory cascades because ion movement is triggered by light and appears to be coupled directly to photosynthetic activity. The intermediary steps in signal transduction are not well understood and are part of the focus for this project. The transduction of these signals can provide important clues to mechanisms by which plant cells regulate transport at the plasma membrane.