Long and Medium-Term Research: In Situ Analysis of the Distribution and Phylogeny of Bacteria Isolated from Deep- Sea Hydrothermal Vents

中长期研究：深海热液喷口分离细菌分布和系统发育的原位分析该奖项由国外卓越中心长期和中期研究项目资助。它使美国科学家和工程师能够在国外公认的卓越研究中心进行三到十二个月的研究。该计划的奖励为联合研究提供了机会，并利用独特或互补的设施、专业知识和国外的实验条件。该奖项将支持俄勒冈州立大学的S. Craig Cary博士对法国海洋资源开发研究所（IFREMER）进行为期三个月的博士后研究访问，与Daniel Desbruyeres博士合作。深海热液喷口的发现揭示了一个独特的海洋无脊椎动物群落，这些无脊椎动物完全依靠化学自养内共生细菌固定的还原碳为生。这些深海喷口的有限地理位置和地球化学上相似的栖息地使人们对自由生活的细菌的多样性和分布产生了有趣的疑问。在IFREMER建立了从一系列热液和冷渗地点分离的前所未有的细菌收集。卡里博士和德斯布鲁耶尔博士将研究深海细菌在热液和冷渗栖息地内部和之间的系统发育和分布。从不同的地理位置和深度范围分离的自养细菌将被检查。16S核糖体RNA序列分析将用于确定细菌的遗传多样性和亲缘关系。将这些序列与已建立的序列数据库进行计算机比较，将允许系统发育分析来确定微生物群落的结构。与管栖喷口多毛体Alvinella pompejana相关的细菌的遗传多样性也将被确定，并与自由生活和内共生的代表进行比较。奖励建议提供国际旅费和三个月的津贴。

Long & Medium-Term Research: In Situ Analysis of the Distribution and Phylogeny of Bacteria Isolated from Deep-Sea Hydrothermal Vents This award is under the Long and Medium-Term Research at Foreign Centers of Excellence Program, which enables U.S. scientists and engineers to conduct three to twelve months of research abroad at research centers of proven excellence. The program's awards provide opportunities for joint research, and the use of unique or complementary facilities, expertise and experimental conditions abroad. This award will support a three-month postdoctoral research visit by Dr. S. Craig Cary of Oregon State University to the Institut Francais de Recherche pour L'Exploitation de La Mer (IFREMER) to work with Dr. Daniel Desbruyeres. The discovery of the deep-sea hydrothermal vents revealed a unique community of marine invertebrates solely supported on reduced carbon fixed by chemoautotrophic endosymbiotic bacteria. The restricted geography of these deep-sea vents and geochemically similar habitats lends itself to interesting questions concerning the diversity and distribution of the free-living bacteria. An unprecedented collection of bacterial isolates from a range of hydrothermal and cold-seep sites has been established at IFREMER. Dr. Cary and Dr. Desbruyeres will examine the phylogeny and distribution of deep-sea bacteria within and between hydrothermal and cold-seep habitats. Isolates of autotrophic bacteria from different geographic locations and depth ranges will be examined. 16S ribosomal RNA sequence analyses will be used to determine the genetic diversity and relationship of the bacteria. Computer comparisons of these sequences with established sequence data bases will allow phylogenetic analysis to define the structure of the microbial community. The genetic diversity of bacteria found associated with the tube-dwelling vent polychaete Alvinella pompejana will also be ascertained and compared to the free-living and endosymbiotic representatives. The award recommendation provides funds to cover international travel and a stipend for three months.