Microbial Genome Sequencing: Genomic Sequencing of Two Globally Ubiquitous Members of the Epsilon Proteobacteria Found in Deep-Sea Hydrothermal Environments

博士们已获得一笔赠款。特拉华大学海洋研究学院的克雷格·加里和芭芭拉·坎贝尔对Am-H的基因组进行了测序，Am-H是一种自由生活的细菌，代表了深海热液喷口和深层地下生物圈中发现的主要细菌类型。此外，我们还将与Lucigen公司合作进行一项试点研究，以证明我们有能力从一种细菌的单细胞中对基因组进行测序，这种细菌与深海热液喷口环节动物Alvinella pompejana有共生关系。这两种目标细菌都属于epsilon变形菌门。除了人类肠道病原菌幽门螺杆菌和弯曲杆菌外，没有对环境相关的epsilon变形杆菌进行测序。这两种海洋细菌在生理上不同于这些人类病原体，它们固定二氧化碳以进行新陈代谢。它们通过一种鲜为人知的替代碳固定途径来完成这项任务。研究人员将使用目前特拉华大学和基因组研究所（TIGR）可用的标准方法对Am-H基因组进行测序。来自这些细菌的基因组序列信息将扩大研究人员对这些全球普遍存在的细菌的广泛研究。研究这些基因组还将帮助研究人员了解细菌的进化，甚至是地球上生命的早期历史。本研究将促进对全球碳循环和二氧化碳固定过程感兴趣的研究人员的知识。最后，单细胞基因组技术的分支将对环境基因组学的未来产生重大影响。直接地说，这项研究将为环境基因组学和生物信息学的研究生和本科生提供极有价值的培训。该项目也将成为特拉华大学在国内和国际中学推广项目的重点，该项目在过去三年的运营中直接惠及了10万多名学生。(www.ocean.udel.edu/expeditions/index.html)。该项目产生的序列将提交给两个公共数据库，并将以演示和出版形式呈现。

A grant has been awarded to Drs. Craig Cary and Barbara Campbell of the University of Delaware College of Marine Studies to sequence the genome of Am-H, a free-living bacterium that represents the dominant type of bacteria found at deep-sea hydrothermal vents and the deep subsurface biosphere. In addition, a pilot study will be undertaken in collaboration with Lucigen Corporation to prove our ability to sequence a genome from a single cell of a bacterium found in a symbiotic relationship with the deep-sea hydrothermal vent annelid, Alvinella pompejana. Both target bacteria belong to the epsilon Proteobacteria. No environmentally relevant epsilon Proteobacteria have been sequenced except the human intestinal pathogens, Helicobacter and Campylobacter. Both marine bacteria are physiologically distinct from these human pathogens, where they fix carbon dioxide for their metabolism. They perform this task via a poorly described alternative carbon fixation pathway. The investigators will sequence the Am-H genome to completion using standard methods currently available at the University of Delaware and at The Institute for Genome Research (TIGR). Genome sequence information from these bacteria will expand the research of a wide range of investigators working on these globally ubiquitous bacteria. Studying these genomes will also help researchers to understand bacterial evolution and even the early history of life on Earth. This study will advance the knowledge of researchers interested in global carbon cycling and carbon dioxide fixation processes. Finally, the ramifications of single cell genomic technology will make a substantial impact on the future of environmental genomics. Directly, this research will provide extremely valuable training for both graduate and undergraduate students in environmental genomics and bioinformatics. This project will also be a focus of the University of Delaware's national and international outreach program for secondary schools that has directly reached over 100,000 students in the last 3 years of operation. (www.ocean.udel.edu/expeditions/index.html). Sequences resulting from this project will be submitted to two public databases and will be presented in both presentation and publication form.