Microbial Genome Sequencing: Genomic Sequencing of Two Globally Ubiquitous Members of the Epsilon Proteobacteria Found in Deep-Sea Hydrothermal Environments

微生物基因组测序：深海热液环境中发现的两个全球普遍存在的 Epsilon Proteobacteria 成员的基因组测序

• 批准号: 0333203
• 负责人: Stephen Cary
• 金额: --
• 依托单位: University of Delaware
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0333203&HistoricalAwards=false
• 关键词: Microbial; Genome; Sequencing; Genomic; Two

A grant has been awarded to Drs. Craig Cary and Barbara Campbell of the University of Delaware College of Marine Studies to sequence the genome of Am-H, a free-living bacterium that represents the dominant type of bacteria found at deep-sea hydrothermal vents and the deep subsurface biosphere. In addition, a pilot study will be undertaken in collaboration with Lucigen Corporation to prove our ability to sequence a genome from a single cell of a bacterium found in a symbiotic relationship with the deep-sea hydrothermal vent annelid, Alvinella pompejana. Both target bacteria belong to the epsilon Proteobacteria. No environmentally relevant epsilon Proteobacteria have been sequenced except the human intestinal pathogens, Helicobacter and Campylobacter. Both marine bacteria are physiologically distinct from these human pathogens, where they fix carbon dioxide for their metabolism. They perform this task via a poorly described alternative carbon fixation pathway. The investigators will sequence the Am-H genome to completion using standard methods currently available at the University of Delaware and at The Institute for Genome Research (TIGR). Genome sequence information from these bacteria will expand the research of a wide range of investigators working on these globally ubiquitous bacteria. Studying these genomes will also help researchers to understand bacterial evolution and even the early history of life on Earth. This study will advance the knowledge of researchers interested in global carbon cycling and carbon dioxide fixation processes. Finally, the ramifications of single cell genomic technology will make a substantial impact on the future of environmental genomics. Directly, this research will provide extremely valuable training for both graduate and undergraduate students in environmental genomics and bioinformatics. This project will also be a focus of the University of Delaware's national and international outreach program for secondary schools that has directly reached over 100,000 students in the last 3 years of operation. (www.ocean.udel.edu/expeditions/index.html). Sequences resulting from this project will be submitted to two public databases and will be presented in both presentation and publication form.

特拉华州大学海洋研究学院的克雷格卡里博士和芭芭拉坎贝尔博士获得了一笔赠款，用于对Am-H的基因组进行测序，Am-H是一种自由生活的细菌，代表了在深海热液喷口和深层地下生物圈发现的主要细菌类型。 此外，还将与Lucigen公司合作进行一项试点研究，以证明我们有能力对与深海热液喷口环节动物Alvinella pompejana有共生关系的一种细菌的单个细胞进行基因组测序。 这两种目标细菌都属于变形菌门。 除了人类肠道病原体，螺杆菌和弯曲杆菌外，没有环境相关的变形杆菌被测序。 这两种海洋细菌在生理上与这些人类病原体不同，它们在那里固定二氧化碳进行新陈代谢。 他们通过一个描述不佳的替代碳固定途径来完成这项任务。 研究人员将使用特拉华州大学和基因组研究所（TIGR）目前可用的标准方法完成Am-H基因组测序。来自这些细菌的基因组序列信息将扩大对这些全球普遍存在的细菌的广泛研究。 研究这些基因组还将有助于研究人员了解细菌的进化，甚至地球上生命的早期历史。这项研究将提高对全球碳循环和二氧化碳固定过程感兴趣的研究人员的知识。 最后，单细胞基因组技术的分支将对环境基因组学的未来产生重大影响。 这项研究将直接为环境基因组学和生物信息学的研究生和本科生提供极其宝贵的培训。 该项目也将成为特拉华州大学面向中学的国家和国际推广计划的重点，该计划在过去3年的运作中直接接触了10万多名学生。（www.ocean.udel.edu/expeditions/index.html）。该项目产生的序列将提交给两个公共数据库，并将以演示和出版形式呈现。