Phase Fluorometric Sensors

目前最先进的生物过程测量技术是基于过时的技术。迫切需要一种新的通用技术，可以使用共同的原理来测量许多分析物。相荧光法基于一个简单的原理有望弥合这一差距：荧光团的寿命随分析物的变化而变化，当用强度调制光激励探头时，它可以作为相角变化来测量。利用荧光寿命(相位和调制)测量，这个项目将利用化学、光学、以及用于生物反应器中在线pH、溶解氧、溶解二氧化碳和葡萄糖传感的电子设备。

The current state-of-the-art for bioprocess measurements is based on dated technology. A new generic technology that can measure a number of analytes using a common principle is greatly needed. Phase fluorometry promises to bridge this gap based on a simple principle: a fluorophore exhibits a lifetime change in response to an analyte, which can be measured as a phase angle change when the probe is excited with intensity modulated light. Using fluorescence lifetime (phase and modulation) measurements, this project will exploit recent advances in chemistry, optics, and electronics for on-line pH, dissolved oxygen, dissolved carbon dioxide and glucose sensing in bioreactors.