Molecular Biology and Physiology of Neuronal Modulation

有许多递质物质调节这种活动 中枢神经系统的神经细胞。 的目标 这一建议是为了阐明这些机制， 递质物质激活细胞内信使过程 导致兴奋性的调节， 钾通道电导特性。 最近的研究 显示神经元的兴奋性依赖于钾 这些通道反过来又受称为Gi的G蛋白调节， Go，其中有几个子类型。 其具体目的是 研究的目的是确定哪些G蛋白亚型是 与神经递质作用（乙酰胆碱和 生长抑素）。 分子生物学技术将用于 产生特异性突变以选择性地产生IgG蛋白， 从而确定他们中的哪一个直接受到 神经递质作用于细胞的钾传导。 为 这一目的突变的α亚基将被插入AtT-20 细胞，一种含有G蛋白的垂体瘤细胞系 调节钾通道。 突变体的影响 对钾离子通道功能的影响。

There are many transmitter substances which regulate the activity of nerve cells of the central nervous system. The objective of this proposal is to elucidate the mechanisms by which these transmitter substances activate intracellular messenger processes that lead to the modulation of excitability through their action on potassium channel conductance properties. Recent studies have shown that excitability of neurons is dependent on potassium channels which are, in turn, regulated by G-proteins called Gi and Go, of which there are several subtypes. The specific aim of this investigation is to identify which of the G-protein subtypes are related to neurotransmitter action (acetylcholine and somatostatin). Molecular biological techniques will be used to produce specific mutations to selectively inactivate G-proteins and thereby determine which of them is directly affected by the neurotransmitters to act on potassium conductance of the cell. For this purpose mutated alpha subunits will be inserted into AtT-20 cells, a pituitary tumor cell line which contain G-protein regulated potassium channels. The effect of the mutants can then be ascertained on potassium channel function.