SGER: Direct Sequencing of Albumin

本研究建议收集直接序列信息 来自脊椎动物血清白蛋白基因的编码部分。 最初，将从三只青蛙身上提取DNA（基因）并进行纯化和克隆。 一旦获得这三个人的血清白蛋白DNA序列， 物种，序列将进行比对，以便完整的区域 可以识别相似性。 如果这些相同的区域可以 发现，引物将被开发，以允许扩增 与其他青蛙的白蛋白基因同源 发展这种 引物将允许快速扩增和测序， 一个主要的核基因从大量的青蛙物种。 这 信息在评估 主要的生物群体，广泛用于研究 遗传学与发展

This study proposes to collect direct sequence information from the coding portion of the gene for vertebrate serum albumin. Initially DNA (genes) from three frogs will be purified and cloned. Once serum albumin DNA sequences are obtained for these three species, the sequences will be aligned so that regions of complete similarity can be identified. If such identical regions can be found, primers will be developed to allow the amplification of homologous albumin genes from other frogs. The development of such primers will permit the rapid amplification and sequencing of this major nuclear gene from a large number of frog species. This information can be invaluable in assessing the relationships of major groups of organisms which are used extensively in studies of genetics and development.