Acquisition of Equipment for Isolation of Recombinant Proteins

分子克隆技术的进步使得在微生物中产生大量不同的蛋白质成为可能，以便研究这些蛋白质的生物学和物理性质。弗吉尼亚大学生物化学系在各种生物系统的生物物理研究方面有着悠久的历史，其中几个教职员工，特别是最近招聘的教职员工，都有围绕这种重组蛋白质的研究设计的研究项目。在13名全职教师中，有8名正在使用重组蛋白或计划在不久的将来使用它们。这种产生研究材料的方法的一个问题是，在组装从细菌或其他类型的细胞中分离这些蛋白质所需的硬件方面。我们在细菌学或大规模蛋白质纯化方面没有很长的历史，所以我们的资源曾经足以满足我们的生化需求，现在完全不足以产生重组蛋白质。许多预计的生物物理研究，特别是需要大量蛋白质的事实，使这一不足变得更加严重。我们建议购买从大肠杆菌、酵母和其他细胞中中等规模分离重组蛋白的基本仪器。

Advances in molecular cloning technologies have made it possible to generate large quantities of diverse proteins in micro-organisms, in order to study the biological and physical properties of these proteins. The Department of Biochemistry at the University of Virginia has a long history of biophysical studies on diverse biological systems and several of the faculty, particularly the faculty recently recruited, have research projects designed around studies of such recombinant proteins. Of the 13 full time faculty, 8 are either using recombinant proteins or plan to use them in the near future. One problem with this approach to the generation of research material is in assembling the hardware necessary for the isolation of these proteins from bacterial, or other types of, cells. We have not had a long history of bacteriology or large scale protein purification and so our resources which were once adequate for our biochemical needs are now completely inadequate for generation of recombinant proteins. This shortfall is compounded by the fact that for many of the projected biophysical studies particularly large amounts of protein are required. We propose to purchase the basic instrumentation for moderate scale isolation of recombinant proteins from E. coli, yeast, and other cells.