Muscle Development in Squid: An Integrated Analysis of Muscle Fiber Specialization

两种肌纤维类型的发生和分化 将研究乌贼Sepioteuthis lessoniana。 第一、 透射电子显微镜将用于记录 触角横纹肌细胞的分化 和手臂上的斜纹肌细胞。 二是 这两种肌细胞类型的分化将是相关的 在发育和成长过程中发生的行为变化 用高速录像记录猎物的捕获过程。 第三、 免疫细胞化学技术将被用来记录模式 以及α-辅肌动蛋白和副肌球蛋白、蛋白质 重要的是确定的规模和组织的 肌细胞丝 这种肌纤维分化的综合方法将 对头足类动物的肌肉进行了最全面的描述 发展至今。 它将提供一个独特的机会， 两种肌肉条纹类型之间的过渡，并将检查 肌细胞结构特征的意义 专门用于特定的功能。 此外还将 检查控制两个不同的组件的机制 肌肉细胞类型在发展过程中，因此，将提供 对肌肉细胞生长机制的基本见解， 发展 因为这项研究将综合分析几个 组织水平，组织的生理意义， 肌肉的结构和生化成分的多样性可以 精确地探索。

The development and differentiation of two muscle fiber types in the squid Sepioteuthis lessoniana will be studied. First, transmission electron microscopy will be used to document the differentiation of the cross-striated muscle cells of the tentacles and the obliquely striated muscle cells of the arms. Second, the differentiation of these two muscle cell types will be correlated with behavioral changes that occur during development and growth using high speed video recording of prey capture. Third, immunocytochemical techniques will be used to document the pattern and timing of expression of alpha-actinin and paramyosin, proteins important in determining the dimensions and organization of the muscle cell filaments. This integrated approach to muscle fiber differentiation will provide the most comprehensive account of cephalopod muscle development to date. It will afford a unique opportunity to study the transition between two muscle striation types and will examine the significance of the structural characteristics of muscle cells specialized for a particular function. In addition, it will examine the mechanisms controlling the assembly of two distinct muscle cells types during development and will, therefore, provide fundamental insight into the mechanisms of muscle cell growth and development. Because the study will integrate analyses at several levels of organization, the physiological significance of the diversity of structural and biochemical components of muscle can be explored precisely.