Optimization of Heterologous Protein Secretion from YarrowiaLipolytica: An Integrated Biomolecular and Bioprocess Engineering Approach

9308822 Ryu本项目旨在研究酵母脂解耶氏菌的表达和分泌系统。这项工作涉及广泛的分子生物学研究，包括转化-分泌系统的开发、启动子的遗传分析和高分泌突变体的分离。开发的菌株将用于表达具有工业意义的模型蛋白，并将使用两级补料间歇反应器系统优化表达水平。

9308822 Ryu This project is on research to develop an expression and secretion system for the yeast Yarrowia Lipolytica. The work involves extensive molecular biological studies which includes development of a transformation-secretion system, genetic analysis of a promoter, and isolation of a high secretion mutant. The developed strain will be used to express model proteins of industrial relevance, and a two stage fed-batch reactor system will be used to optimize expression levels.