Using Cobalt to Probe DNA and RNA Folding

这个职业发展奖的妇女从有机动力 和特别项目计划支持教授的工作 纽约州立大学斯托尼分校的辛西娅·J·伯罗斯（Cynthia J. Burrows） 布鲁克这项工作涉及到对 使用DNA和RNA的简单钴化合物的氧化反应 作为研究者DNA氧化的延伸 使用镍（II）络合物的研究。特别是DNA序列 含有G-G错配、G凸起或发夹环的基因将被 为了开发这种结构的探测器而进行的研究。 对RNA的研究将包括检查转移RNA和 Ⅰ组内含子。本实验方法 工作将集中在DNA和RNA的高分辨率纯化 凝胶电泳 %%% 授予Burrows教授的职业发展奖 她在镍络合物及其与DNA相互作用方面的工作， 钴配合物和RNA结构操纵和催化。 这些非卟啉配合物对DNA和 RNA在具有双错配的DNA中寻找，转移RNA， Ⅰ组内含子。这项工作预计将影响 医学协调和生物化学

This Career Advancement Award for Women from the Organic Dynamics and Special Projects Programs supports the work of Professor Cynthia J. Burrows at the State University of New York, Stony Brook. The work concerns an investigation into the oxidative reactions of simple cobalt compounds using DNA and RNA targets as an extension of the investigator's DNA oxidation studies using nickel(II) complexes. In particular, DNA sequences containing G-G mismatches, G bulges or hairpin loops will be studied in efforts to develop a probe for such structures. Studies on RNA will include examination of transfer RNA's and the group I intron from Tetrahymena. The experimental methods of this work will focus on DNA and RNA purification by high resolution gel electrophoresis. %%% This Career Advancement Award to Professor Burrows will extend her work on nickel complexes and their interactions with DNA to use of cobalt complexes and to RNA structure manipulations and catalysis. The application of these nonporphyrin complexes to both DNA and RNA is sought in DNA with double mismatches, transfer RNA, and group I intron from Tetrahymena. The work is expected to impact on medical, coordination, and biochemistry.