Dynamic Control of the Binding of Neutral Biomolecules

有机动力学计划的这笔赠款支持以下工作： 史密斯教授在圣母大学研究方法 以调节小分子（氨基酸，核苷， 大分子（酶、糖蛋白）， 非生物的硼酸受体。第一 涉及一系列硼酸的合成和研究， 冠醚分子被设计成异位结合， 共转运水溶性二醇配体和金属阳离子 亲脂膜这种方法是基于离子强度 控制绑定。第二种方法涉及光开关 受体，并基于光化学控制的结合 的生物分子。合成受体是亲脂性的， 光开关硼酸偶氮苯并咪唑载体分子 它也将被探索为光能跨膜质子 泵. %%% 在这个项目中，史密斯教授将研究 调节各种类型的生物配体与 非生物硼酸受体离子强度 控制和光控制的结合方法将是 研究了在基于离子的方法中， 异位受体由金属离子控制，而 光控制方法涉及光可转换受体。这 研究可能具有技术应用，例如 （生物）化学净化装置，运输载体 通过亲脂膜和控释材料。

This grant from the Organic Dynamics Program supports the work of Professor Smith at the University of Notre Dame to study methods to regulate the binding of small (amino acids, nucleosides, saccharides) and large (enzymes, glocoproteins) biomolecules to abiotic, boronic acid-based receptors by two avenues. The first involves the synthesis and study of a series of boronic acid crown ether molecules designed to heterotopically bind and cotransport water-soluble diol-ligands and metal cations across lipophilic membranes. This approach is based on ionic strength controlled binding. The second approach involves photoswitchable receptors and is based on the photochemically controlled binding of the biomolecules. The synthetic receptors will be lipophilic, photoswitchable boronic acid azobenzenediol carrier molecules which will also be explored as light-powered transmembrane proton pumps. %%% In this project Professor Smith will investigate methods of regulating the binding of various types of biological ligands to abiotic, boronic acid-based receptors. Both ionic strength controlled and light controlled binding methods will be investigated. In the ionic based method the binding to the heterotopic receptors is controlled by metal ions, while the light controlled method involves photoswitchable receptors. This research may have technological applications such as (bio)chemical purification devices, carriers for transport through lipophilic membranes, and controlled release materials.