Effect of Electroporation on Permeation of Cryoprotectants and Water Across Membranes of Biological Cells

9311906皮特这个为期一年的探索性项目正在进行研究，以确定强电场对水和低温保护剂(CPA)跨细胞膜渗透的影响。电穿孔对膜通透性的影响，将通过对模型细胞系统和单一CPA(甘油)的测定来探索基本概念和阐明机制。该项目将提供初步数据，将涉及：(1)设备的组装和测试；(2)研究电穿孔条件对细胞活力的影响；(3)量化不同电穿孔条件下甘油对细胞的渗透率。这项研究的结果可能为在低温下长期保存活细胞提供重要信息。这在生物技术、农业和医学中都有应用。***

9311906 Pitt This one year exploratory project is on research to determine the effect of strong electric fields on the permeation of water and cryoprotectants (CPAs) across cell membranes. Membrane permeabilities, as affected by electroporation, will be determined for a model cell system and a single CPA (glycerol) to explore the basic concepts and elucidate mechanisms. This project, which is to provide preliminary data, will involve: (1) the assembly and testing of equipment; (2) a study of the effect of electroporation conditions on cell viability; and (3) experiments to quantify the penetration rate of glycerol into cells at different electroporation conditions. The results of this research may provide important information for the long - term storage of viable cells at cryogenic temperatures. This has applications in biotechnology, agriculture and medicine. ***