SBIR Phase II: Reversed Micellar Fractionation of Recombinant-DNA Therapeutic Protein Intermediates

9313977蓖麻反胶束作为一种从溶液混合物中进行选择性液液萃取蛋白质的方法具有很大的前景，这样可以获得比原始饲料更纯、更浓缩的蛋白质。反胶束-有机(RM-ORG)相分离在生物合成蛋白质生产过程的初步纯化或初始分级过程中具有重要的应用价值。在第一阶段开发的连续流动RM-ORG工艺旨在取代导致产量损失和制造成本增加的传统低分辨率层析纯化工艺。在第二阶段中，需要优化表面活性剂浓度和水溶液pH值等工艺流程参数。这些参数在决定蛋白质在反胶束中的增溶作用中起着关键作用。将设计、建造和测试一个连续流RM-ORG原型。根据这些信息，将对原型进行经济分析。

9313977 Castor Reversed micelles hold much promise as a means of carrying out selective liquid-liquid extraction of proteins from solution mixtures such that the desired protein is obtained in a purer and more concentrated form than the original feed. Reversed micellar-organic (RM-ORG) phase separation could have significant utility in the primary purification or initial fractionation of biosynthetic protein production processes. The continuous flow RM-ORG process developed during Phase I was designed to displace conventional low resolution chromatographic purification processes which cause losses in yield and increased manufacturing costs. During Phase II, process stream parameters such as surfactant concentration and aqueous stream pH are to be optimized. These parameters play key roles in determining protein solubilization in reversed micelles. A continuous flow RM-ORG prototype is to be designed, constructed, and tested. Based on this information, an economic analysis of the prototype is to be performed.