SGER: Iron Fertilization of Oceanic Phytoplankton: A Molecular of Chloroplast Gene Response

铁在维持细胞代谢中起着关键作用， 浮游植物似乎调节细胞内铁浓度。 然而，目前还没有人对这种蛋白质进行分子研究。 铁剥夺对编码蛋白质的基因的影响 光系统II、卡尔文循环酶和光捕获 叶绿素 这个项目将确定叶绿体基因 在真核海洋生物中， 学名：Olisthodiscus luteus. 这是一个生态上 在有毒藻类大量繁殖中具有重要意义， 分子水平，将作为一个模型系统，以解决关键 其他海藻的生理和生态问题。 的 这项工作将提供关于特定基因如何 在转录或转录后调节这些蛋白质 铁作为一种环境线索。 如果成功， 未来的工作将集中在施加转录控制的因素上 或影响RNA稳定性和/或选择性利用的那些 特定的RNA种类。

Iron plays a critical role in the maintenance of cell metabolism as phytoplankton appear to regulate intracellular iron concentrations. However, at present no one has conducted molecular studies on the effects of iron deprivation on the genes that encode proteins of photosystem II, Calvin cycle enzyme, and light harvesting chlorophyll. This project will determine how chloroplast gene expression is regulated by iron (Fe++) in the eukaryotic marine alga, Olisthodiscus luteus. This alga, which is ecologically significant in toxic algal blooms yet essentially unstudied at the molecular level, will serve as a model system to address critical physiological and ecological questions in other marine algae. The work will provide critical information on how specific genes regulate these proteins at the transcription or post-transcription level in response to iron as an environmental cue. If successful, future work will focus on factors that impose transcription control or those that influence RNA stability and/or selective utilization of specific RNA species.