An Investigation of the Relationship Between Community Composition and Metabolic Capabilities of Coastal Bacterioplankton Communities

该项目的目标是了解以下关系：1)微生物群落的分类组成；2)它们的代谢能力和特征代谢途径的存在；3)促进浮游细菌生产的有机物来源。为了做到这一点，我们建议继续开发和测试一种基于16S rRNA基因可变区域分析来描述浮游细菌群落结构的技术。我们将通过评估浮游细菌群落在单一碳源上的生长能力和外源胸苷的代谢命运来表征它们的代谢能力。主成分分析的统计程序将用于比较这些群落特征，并开发浮游细菌种群代谢能力和系统发育特征的矩阵。通过比较浮游细菌DNA的C和N稳定同位素特征与来自同一位置的有机物组分的碳和N稳定同位素特征，来评估浮游细菌生产碳的来源。这项工作将在旧金山和托马莱斯湾进行，在旧金山附近的沿海水域，在东海岸的河口和沿海水域进行，为期三年。* * *

Hollibaugh 9315639 The goal of the project is to understand the relationship between: 1) the taxonomic composition of microbial communities; 2) their metabolic capabilities and the presence of characteristic metabolic pathways; and 3) the sources of organic matter fueling bacterioplankton production. To do this, we propose to continue development and testing of a technique for describing bacterioplankton community structure based on an analysis of variable regions of the 16S rRNA gene. We will characterize the metabolic capabilities of bacterioplankton communities by assessing their abilities to grow on single carbon sources and by the metabolic fate of exogenously supplied thymidine. The statistical procedure of principal components analysis will be used to compare these community characteristics and to develop a matrix of the metabolic capabilities and phylogenetic characteristics of bacterioplankton population. The sources of C fueling bacterioplankton production will be assessed by comparing the C and N stable isotope signatures of bacterioplankton DNA with those of organic matter fraction form the same location. The work will be conducted in San Francisco and Tomales Bays, in coastal waters around San Francisco and in estuaries and coastal water off the East coast over a 3 year period. ***