Development of High Resolution X-Ray Microscopy

9316594柯兹扫描软X射线显微镜已在布鲁克海文国家实验室国家同步辐射光源建成并投入使用。该仪器利用X1A型光束线上明亮的、可调谐的波动光源。它使用在劳伦斯伯克利实验室/IBM合作下制造的波带板来形成50 nm的光束斑点。显微镜在常规操作中，由几个小组使用，以光点大小设置的分辨率对潮湿的生物样本进行成像。正在开发新的成像模式，以扩展该技术的适用性，利用元素吸收边(XANES)附近的共振，并使用x射线束激发的荧光标记中的可见光的检测，绘制特定的化学状态图。除了继续操作显微镜外，还要求提供支持以扩展XANES对比度成像能力，大幅提高仪器的稳定性和分辨率，并探索在更高能量下进行X射线显微镜的可能性。该团队欢迎对使用该仪器解决与其独特能力相匹配的生物问题感兴趣的调查人员的询问。U~S和t&amp；9：；=？@A B C D E F G H I J K L M N O P R S T U V W X Y Z\K_`a b C d e f g H i j k r m o I t u v w x y{|~9316594柯兹扫描软X射线显微镜已在国家同步罗恩光源建成并投入运行，B pV x^`b！F b p b 3时代新罗马符号&Amp；Arial 1信使L“He=抽象沙漠国王，BIR沙漠国王，BIR

9316594 Kirz A scanning soft x ray microscope has been built and put into operation at the National Synchrotron Light Source, Brookhaven National Laboratory. The instrument makes use of the bright, tuneable undulator source at the X1A beamline. It uses zone plates fabricated under a Lawrence Berkeley Lab./IBM collaboration to form a 50 nm beam spot. The microscope is in routine operation, and is used by several groups to image wet biological specimens at the resolution set by the spot size. New imaging modes are being developed that extend the applicability of the technique to map particular chemical states using the resonances present near elemental absorption edges (XANES), and using the detection of visible light excited in fluorescent labels by the x ray beam. In addition to continuing the operation of the microscope, support is requested to extend the XANES contrast imaging capability, to improve substantially the stability and the resolution of the instrument, and to explore the possibilities in x ray microscopy at higher energies. The team welcomes inquiries by investigators interested in using the instrument to solve biological problems matched to its unique capabilities. u ~ s &t &9 : ; = ? @ A B C D E F G H I J K L M N O P R S T U V W X Y Z \ K _ ` a b c d e f g h i j k r m o I t u v w x y { | ~ 9316594 Kirz A scanning soft x ray microscope has been built and put into operation at the National Synchrot ron Light Source, b p v x ^ ` b ! ! ! ! F b p b 3 Times New Roman Symbol & Arial 1 Courier L " h e e = abstract Deseree King, BIR Deseree King, BIR