Acquisition of a Confocal Microscope for the Levine Science Resource Center

该建议是一个多用户共焦显微镜和配套仪器。 杜克大学的核心用户群包括化学系、动物学系、细胞生物学系、免疫学系以及细胞生长、调节和癌症系的教师。 1994年，一座新的建筑物，即科学资源中心，将投入使用，该中心目前正在作为一个多学科科学设施建造。 该建筑将容纳环境学院，以及工程学院，艺术与科学学院和医学院的组成部分。 该建筑的核心设施之一将是显微成像中心。 该设施以及大楼中所有其他共享用户技术的目的是使硬件和软件保持在生物技术的前沿，并找到将这些技术与其他新兴领域的进步相结合的方法。 新大楼还计划建设一个核磁共振中心、X射线晶体学和蛋白质建模中心以及该大学的转基因小鼠中心。 每个中心将共享用户操作，这些操作将相互补充，并将与显微成像中心接口。 这些中心将促进新的发展，因为化学家，生物化学家，物理学家，生物化学家，生物学家和计算机科学家在这些技术的持续发展中相互作用。 这个共聚焦显微镜的应用程序汇集了来自五个部门的七名教师作为核心用户。 我们每个人都使用过共焦显微镜，无论是在其他机构（我们中的四个人是杜克的新教师），或在杜克。 目前中心有一台使用量越来越大的共焦显微镜。 该显微镜需要升级，它本身不再能够支持所提出的许多应用。 因此，这项请求是为了部分支持现有显微镜的升级工作，并购买新一代的共焦显微镜以及支持硬件和软件。 我们的项目相当多样化。 新的创新，加上用户流量的增加，将需要一个新的显微镜平台和新的可用激光源，再加上将内置到新建筑中的网络功能，这些都将得到促进。

This proposal is for a multi-user confocal microscope and supporting instrumentation. The core user group at Duke University include faculty in the Department of Chemistry, Zoology, Cell Biology, Immunology, and the Section of Cell Growth, Regulation and Cancer. In 1994, a new building, the Science Resource Center, now being built as a multidisciplinary science facility, will be occupied. The building will house the School of the Environment, and components of the Schools of Engineering, Arts and Sciences, and Medicine. One of the core facilities in that building will be an microscopic Imaging Center. The intent for that facility, and all other shared-user technologies in the building, is to keep the hardware and software at the leading edge of biotechnology and to find ways to interface these technologies with advances in other emerging areas. Also being planned for the new building are an NMR center, X-ray crystallography and protein modeling Center, and the University's transgenic mouse Center. Each of these Centers will be shared user operations which will complement one another and will interface with the Microscopic Imaging Center. These Centers will foster new developments as the chemists, biophysicists, physicists, biochemists, biologists, and computer scientists interact with one another in the continuing development of these technologies. This application for a confocal microscope brings together seven faculty from five departments as the core users. Each of us has used confocal microscopes, either at other institutions (four of us are new faculty at Duke), or at Duke. At present the Center has one confocal microscope that has been getting increasingly heavier use. That microscope is in need of an upgrade, and it is no longer, by itself, capable of supporting many of the applications that are proposed. Therefore, this request is for partial support of an effort to upgrade the existing microscope and to purchase a newer generation of confoc al microscope plus support hardware and software. Our projects are quite varied. New innovations, plus the increase in traffic of users will require a new microscopic platform and newly available laser sources, plus they will be facilitated by the networking capabilities that will be build into the new building.