Mechanistic Studies of Electroosmotic Control in Capillary Zone Electrophoresis

摘要本项目旨在了解毛细管区带电泳（CZE）的影响因素，以开发梯度洗脱高效毛细管电泳。SYMBOL 122 \f“SYMBOL”电位的大小和极性强烈影响蛋白质吸附以及电渗透流的方向和大小。在这个项目中，将测试径向电位梯度，作为改变SYMBOL 122 \f“SYMBOL”电位的一种手段，从而减少蛋白质吸附并提高该技术的旋转功率。在静压驱动的流体组件下进行分散研究，将在一组改变电渗透控制下毛细管分数长度的试验中进行。结合Gouy-Chapman-Stern-Grahame理论的串联电容模型将用实验结果进行评价。CZE是一种具有电泳分析能力和高效液相色谱分析方便的极具发展前景的技术。它的发展将为支持生物技术和制药工业提供有力的工具。‘ ’ IBMDOS ' ' ' AUTOEXECBAK ‘ ’ Õ FLW K PSFONTS K ！89分钟前。[摘要]本研究的目的是了解资本流动的某些影响因素3个5!！J !F （Times New Roman Symbol & Arial, 99 " h# c %#c % 5）尼克尔·科沃德

CTS-9318694 Iowa State University Cheng S. Lee ABSTRACT The aim of this project is to understand certain factors in capillary zone electrophoresis (CZE) in order to develop gradient elution High Performance Capillary Electrophoresis. The magnitude and polarity of the SYMBOL 122 \f "Symbol" potential strongly influences protein adsorption as well as the direction and magnitude of electroosmotic flow. For this project, a radial potential gradient will be tested as a means of varying the SYMBOL 122 \f "Symbol" potential and thereby reducing protein adsorption and enhancing the revolving power of the technique. Dispersion studies under a hydrostatic pressure-driven flow component will be conducted in a set of tests in which the fractional length of the capillary under electroosmotic control will be changed. A series capacitance model combined Gouy-Chapman-Stern-Grahame theory will be evaluated with experimental results. CZE is a very promising technique that offers the analytical capabilities of electrophoresis and the convenience of HPLC. Its development will provide a powerful tool in support of the biotechnology and pharmaceutical industries. IBMBIO COM' ` IBMDOS COM' ` AUTOEXECBAK s" Õ FLW K PSFONTS K ! 89DECS PRE .N T" AUTOEXECBAT E J BURKA PRE dP #zs SAVERS j " ICONS j " LM Y CTS-9318694 Iowa State University Cheng S. Lee ABSTRACT The aim of this project is to understand certa in factors in capi v w 3 5 ! !J ! F ( Times New Roman Symbol & Arial , 9 9 " h #c %#c % 5 1 Nichelle Coward Nichelle Coward