Analytical Separations of Large Biopolymers through Capillary Electrophoresis

分析和表面化学计划的这笔赠款将支持诺沃特尼教授对脉冲场毛细管电泳法(CE)在多糖和蛋白多糖的分离和表征中的应用的研究。目标是将CE技术扩展到100,000到100,000,000道尔顿范围内的高分子量生物聚合物。被研究的材料将包括大蛋白质、形状特殊的DNA分子、多糖、蛋白多糖和其他生物分子。此外，诺沃特尼教授和他的学生将对控制脉冲电场中大分子迁移率的因素进行基础研究。这项研究将扩展毛细管电泳法的能力，这是一种允许分离复杂大分子混合物中带电成分的方法。其目标是能够分离分子量高达1亿道尔顿的大分子生物分子。为了克服传统毛细管电泳法分离大分子混合物时遇到的许多问题，将对脉冲电场进行研究。被研究的物质包括多糖、碳水化合物和蛋白多糖。该项目开发的技术将用于分离具有生物化学和技术意义的大分子。

This grant from the Analytical and Surface Chemistry Program will support Professor Novotny's investigations of the applications of pulsed-field capillary electrophoresis (CE) in the separation and characterization of polysaccharides and proteoglycans. The goal is to extend CE technology to high molecular weight biopolymers in the range of 100,000 to 100,000,000 Daltons. The materials to be investigated will include large proteins, DNA molecules with unusual shapes, polysaccharides, proteoglycans, and other biomolecules. In addition, a fundamental investigation of the factors which control the rate of migration of large molecules in pulsed electric fields will be carried out by Professor Novotny and his students. This research will extend the capabilities of capillary electrophoresis, a method that allows the separation of charged components present in complex macromolecular mixtures. The goal is to be able to separate large biomolecules with molecular weights up to 100,000,000 Daltons. Pulsed electric fields will be investigated in order to overcome many of the problems encountered in the fractionation of mixtures of large molecules by conventional capillary electrophoresis methods. The materials to be investigated include polysaccharides, carbohydrates, and proteoglycans. The techniques developed in this project will be used to separate large molecules of biochemical and technological significance