Fluorescence Microscopy for Cell Biology Laboratory

9350778 Prusch冈萨加大学生物系正试图增加和加强新修订和扩展的细胞生物学课程的实验室组成部分，并增加本科生的研究机会。原来高年级一学期的细胞与分子生物学课程已经扩展到两个学期，现在是大二生物专业的必修课。这门扩展细胞生物学课程的实验部分将向学生介绍细胞生物学中使用的各种技术，并强化课程课堂部分介绍的概念。荧光显微镜是细胞生物学中常用的最基本也是最重要的技术之一。荧光显微镜可用于实时跟踪活细胞中的各种事件，以及固定细胞中特定物质的分布。例如，学生将能够研究活细胞中的内吞作用，从信号与细胞表面受体的初始相互作用到内吞体或吞噬体的形成。细胞骨架重排响应环境刺激和局部生化事件在活细胞将被研究。获得荧光显微镜将使生物学学生在实验室中获得新的体验，并大大拓宽了在他们自己的研究项目中使用现代技术的机会。＊＊＊

9350778 Prusch The Biology Department at Gonzaga University is attempting to both increase and strengthen the laboratory component of a newly-revised and expanded Cell Biology course and to increase research opportunities for undergraduate majors. The former upper division one semester Cell and Molecular Biology course has been expanded to two semesters and is now directed at sophomore level biology majors. The laboratory component of this expanded Cell Biology course will introduce students to a variety of techniques used in cell biology as well as reinforce concepts introduced in the lecture portion of the course. One of the most basic as well as important techniques used routinely in cell biology is fluorescence microscopy. Fluorescence microscopy can be used to follow a wide variety of events in living cells in real time, as well as the distribution of specific substances in fixed cells. For example, students will be able to study endocytosis in living cells from the initial interaction of a signal with receptors on the cell surface to endosome or phagosome formation. Cytoskeletal rearrangements in response to environmental stimuli and localized biochemical events in living cells will be studied. Acquisition of fluorescence microscopes will give biology students new experiences in their laboratories and greatly broaden the opportunities for the use of modern technology in their own research projects. ***