Palladium-containing Artificial Peptidases

9404971 Kostic爱荷华州州立大学爱荷华州州立大学化学系的Nenad Kostic博士得到无机、生物无机和有机化学计划的支持，用于开发Pd（II）络合物作为选择性裂解蛋白质的新型无机化学试剂。Kostic已经发现，肽和蛋白质的区域选择性水解是由Pt（II）和Pd（II）络合物促进的。在这个项目中，这种分裂的机制将被研究和分析的金属离子的作用。在这些研究中获得的信息将用于增加裂解反应的选择性和设计将催化裂解的试剂。 将大蛋白质选择性切割成明确定义的较小部分在分析生物化学和生物技术中是重要的。在目前的实践中，一组被称为蛋白酶的酶被用于进行该操作。然而，蛋白酶不切割折叠的蛋白质，并且它们产生许多相对短的蛋白质片段，而通常需要大的片段。本研究旨在用基于金属铂和钯的新型试剂克服这些问题。这个项目已经在生物制药研究中显示出了希望，其中蛋白质的选择性切割已经导致了潜在的商业产品。

9404971 Kostic Iowa State Univ. Dr. Nenad Kostic, Chemistry Department, Iowa State University, is supported by the Inorganic, Bioinorganic, and Organometallic Chemistry Program for the development of Pd(II) complexes as new inorganic chemical reagents for selective cleavage of proteins. Kostic has discovered that regioselective hydrolysis of peptides and proteins is promoted by Pt(II) and Pd(II) complexes. In this project the mechanism of this cleavage will be studied and the role of the metal ion analyzed. The information obtained in these studies will be used to increase the selectivity of the cleavage reactions and to design reagents which will perform the cleavage catalytically. Selective cleavage of large proteins into well defined smaller parts is important in analytical biochemistry and biotechnology. In current practice a group of enzymes known as proteases are used to perform this operation. However, the proteases do not cleave folded proteins and they generate many relatively short pieces of the protein, whereas large segments are often desired. This research is aimed at overcoming these problems with a new type of reagent based on the metals, platinum and palladium. Already this project has shown promise in biopharmaceutical research, where the selective cleavage of a protein has led to a potential commercial product.