Design of Low Molecular Weight Displacers for Protein Purification by Ion Exchange Displacement Chromatography

9412737克雷默本项目致力于研究通过置换层析同时浓缩和纯化蛋白质的低分子置换剂的设计，并将重点研究置换剂化学与蛋白质置换效果之间的关系。该项目包括用确定的蛋白质混合物进行实验，以检验聚合物合成置换剂对动态亲和力的影响。还将研究诱导盐梯度对浓缩能力的影响。一个主要问题将是确定如何在设计足够亲和力的同时不产生异常高的诱导盐梯度。此外，还将审查操作问题，如从受污染区域移除的容易程度和柱的再生。最后，实验将测试本研究中开发的置换物从复杂生物混合物中提纯蛋白质的能力。长期目标是促进这种用于工业生物分离的新技术的实施。***

9412737 Cramer This project is on research to study the design of low molecular weight displacers for the simultaneous concentration and purification of proteins by displacement chromatography, and will focus on the relationships between displacer chemistry and efficacy for protein displacement. Included in this project are experiments with defined protein mixtures to examine the effects of the polymer synthesis of displacers on dynamic affinity. The effects of induced salt gradients on the ability to concentrate will also be studied. A major issue will be to determine how to design for sufficient affinity while not producing inordinately high induced salt gradients. Also, operational issues such as ease of removal from contaminated zones and column regeneration will be examined. Finally, experiments will test the ability of the displacers developed in this research to purify proteins from complex biological mixtures. The long term goal is to facilitate the implementation of this new technology for industrial bioseparations. ***