Displacement Chromatography of Proteins: Identification of Critical Displacer Properties and Development of Novel Nontoxic Displacers

该项目研究置换色谱法和聚合物 综合考察临界置换剂的作用 使用定义明确的聚合物置换剂化合物的性质。 将合成各种新型的聚合物驱替剂 用于离子交换、疏水相互作用和金属 亲和层析系统。 已开发的置换剂 将被纯化并进行适当的分析 化学技术来确定其结构和纯度。 这些化合物的色谱行为将是 使用洗脱色谱法和吸附法评价 等温线将通过前沿色谱法测定。 模型蛋白质饲料混合物显示出一系列的 将确定这些吸附剂系统的性能。 不同分子量的聚合物驱替剂的驱替能力 重量、官能团密度和构象， 在这些系统中作为蛋白质置换剂的作用将被检查， 这些属性的相对重要性 确立了习 置换剂性质的结果数据 将用于识别置换剂的组合 高效生产所需的物理化学性质 生物聚合物的置换和快速去除 用于固定相再生的置换器 材料. 这项基础研究将提供基础 深入了解分子位移现象 水平，并将建立指导方针的设计和 合成用于未来生物分离的合适置换剂 问题

The project studies displacement chromatography and polymer synthesis to investigate the effect of critical displacer properties using well defined polymeric displacer compounds. A variety of novel polymeric displacers will be synthesized for ion exchange, hydrophobic interaction, and metal affinity chromatographic systems. The developed displacers will be purified and subjected to appropriate analytical chemical techniques to establish their structure and purity. Chromatographic behavior of these compounds will be evaluated using elution chromatography and adsorption isotherms will be determined by frontal chromatography. Model protein feed mixtures exhibiting a spectrum of properties will be established for these adsorbent systems. The ability of polymeric displacers of various molecular weights, functional group densities, and conformations to act as protein displacers in these systems will be examined, and the relative importance of these properties will be established. The resulting data on displacer properties will be used to identify combinations of displacer physicochemical properties which are required for efficient displacement of biopolymers and rapid removal of the displacers for regeneration of the stationary phase materials. This fundamental research will provide basic insight into the displacement phenomenon at the molecular level and will establish guidelines for the design and synthesis of appropriate displacers for future bioseparation problems.