Phase Fluorometric Sensors

9413262 Govind该项目正在研究开发一种基于合成荧光团和相移荧光法的新型生物传感器。该方法使用的原理是，当被强度调制的光束激发时，荧光团的发射随着所讨论的分析物的变化而变化。这项研究将侧重于开发可在高频下调制的共振能量转移荧光团和光源。将开发用于杂交瘤细胞培养和微生物发酵的pH、氧气、二氧化碳和葡萄糖的在线传感器并进行测试。***

9413262 Govind This project is on research to develop a new class of biosensors based on synthetic fluorophores and phase-shift fluorometry. The method uses the principle that a fluorophore exhibits shift in its emission in response to the analyte in question when excited by an intensity modulated beam of light. This research will stress the development of resonance energy transfer fluorophores and light sources that can be modulated at high frequencies. On-line sensors for pH, oxygen, carbon dioxide, and glucose will be developed and tested for both hybridoma cell cultures and microbial fermentations. ***