Analysis of the Inverse Effect in Drosophila

9417814将以果蝇的白色位点Birchler为靶基因，研究反式作用剂量调节剂的作用及其与协调基因表达、剂量补偿、非整倍体症状、数量性状和位置效应变异的潜在关系。为了接近最终目标，将克隆和鉴定剂量敏感调节器的例子，描述这种剂量敏感调节器与男性特定致死基因之间的相互作用，并寻找修改白色基因的隐性突变。在多细胞生物体中，基因表达的调控不是一个简单的线性机制。已经或正在描述许多不同的机制。在过去的几年里，该实验室分析了一个调节网络，该网络对它们所影响的基因的表达产生了相反的剂量效应，这一分析将继续下去。***

9417814 Birchler The white locus of Drosophila melanogaster will be used as a target gene to study the action of trans-acting dosage modifiers and their potential involvement with coordinate gene expression, dosage compensation, aneuploid syndromes, quantitative traits and position effect variegation. As an approach to the ultimate goal, examples of dosage sensitive regulators will be cloned and characterized, interactions between such dosage sensitive regulators and the male specific lethal locus characterized and a search for recessive mutations which modify the white gene will be made. %%% The regulation of gene expression in multicellular organisms is not a simple linear mechanism. A number of different mechanisms have been or are being described . Over the past several years a regulatory network that results in an inverse dosage effect on the expression of the genes which they affect has been analyzed by this laboratory and this analysis will be continued. ***