Genetic Analysis of Radiation Response in Plants

9418391将进行拟南芥辐射敏感突变体的遗传分析。相关的基因将被映射，包括物理和遗传。uvh1和uvh6的第二个抑制因子将被定位，并寻找其他抑制因子。 感兴趣的基因将被克隆和测序。遗传重组是植物抗辐射的重要机制这一假设将通过遗传杂交测定两个辐射敏感突变体uvh 1和rad 3中自发的有丝分裂和减数分裂重组的频率来检验。将以各种方式分析辐射敏感突变体中的UV损伤修复类型。 对于植物中DNA损伤的修复机制知之甚少。它们经常暴露在阳光中的紫外线下，这会损害它们的DNA，并且已知它们具有独特的机制来避免和修复这种损伤。一个行之有效的遗传方法将采取继续调查的DNA修复拟南芥，一种模式植物。将利用分子遗传学的现代工具对这种生物的DNA修复基因进行作图、克隆、测序和生化功能分析。 ***

9418391 Mount Genetic analysis of radiation sensitive mutants of Arabidopsis will be done. The involved genes will be mapped, both physically and genetically. Second site suppressors of the uvh1 and uvh6 will be mapped and additional suppressors sought. The genes of interest will be cloned and sequenced. The hypothesis that genetic recombination is an important mechanism of radiation resistance in plants will be tested by determining frequencies of spontaneous mitotic and meiotic recombination in two radiation sensitive mutants, uvh1 and rad3 by genetic crosses. The type of UV damage repair will be analyzed in radiation sensitive mutants in various ways. %%% Very little is known about mechanisms for repair of DNA damage in plants. They are constantly exposed to ultraviolet light in sunlight which damages their DNA and it is known that they have unique mechanisms for avoiding and repairing such damage. A proven genetic approach will be taken to continue an investigation of DNA repair in Arabidopsis, a model plant. The DNA repair genes from this organism will be mapped, cloned, sequenced and analyzed for biochemical function using the modern tools of molecular genetics. ***