Analytical and Preparative Biochemical Equipment

这项申请的目的是更换该部共用仪器设施的旧设备和更新陈旧设备。每一个研究者都参与了纯蛋白质和核酸的制备以及它们的性质、结构和功能的研究。这些纯化需要低速和高速离心。大多数还涉及将生物分子的复杂混合物分离成纯组分。最近的技术创新使这种分离更快、更简单。为此，我们需要一种具有合适检测器的灌注色谱仪。许多活性测量和大多数测序反应涉及放射性测定。为此，我们请求提供一台闪烁计数器。 所有工具将在共享的基础上使用。经验表明，这是使用现代仪器的最具成本效益的方式。 一旦保修期满，个人用户将为这些仪器的维修合同作出贡献。共用维修合同也是使用设备的最具成本效益的方式。

The objective of this application is to replace inoperative equipment and to upgrade obsolescent equipment of the shared instrumentation facility of the department. Each of the investigators on this application is involved in the preparation of pure proteins and nucleic acids and the study of their properties, structure, and function. These purifications require both low and high speed centrifugations. Most also involve the separation of complex mixtures of biomolecules into pure constituents. Recent technological innovations have made such separations much faster and simpler. To this end, we are requesting a perfusion chromatography apparatus with suitable detectors. Many of the activity measurements and most of the sequencing reactions involve radioactive assays. To this end, we are requesting a scintillation counter. All instruments will be used on a shared basis. Experience shows this to be the most cost-effective way to use modern instrumentation. Individual users will contribute to the maintenance contracts of these instruments once the warranties expire. The sharing of maintenance contracts is also the most cost-effective way of using the equipment.