Responses of Antarctic Phytoplankton to Small-Scale Changes in Temperature

9420085夏皮罗过去的报告表明，南极浮游植物对微小的温度变化非常敏感。 然而，由于与当前可用方法相关的问题，实际记录是困难的。 记录细胞死亡和/或代谢停滞，与温度的变化小于与海冰的形成和消退，可能会影响我们对南极生态的理解。 本项目研究了一些最近开发的荧光探针在生物医学研究中的潜在应用，用于评估哺乳动物细胞生物学和生物海洋学，即， 浮游植物代谢活动和细胞死亡。 然而，荧光探针可能会或可能不会被证明是有用的浮游植物细胞的生理状态，以评估温度胁迫。 如果制定出适当的协议，生物海洋学家将能够检查和评估极地浮游植物对极地海冰形成和消退的温度反应。

9420085 Shapiro Past reports suggest that Antarctic phytoplankton are hypersensitive to small thermal changes. However, actual documentation is difficult because of problems associated with currently available methods. Documentation of cell death and/or metabolic arrest, associated with changes in temperature less than that associated with formation and retreat of sea ice, could affect our understanding of Antarctic ecology. This project investigates the potential application of some recently developed fluorescent probes in biomedical research for evaluating mammalian cell biology to biological oceanography, i.e., phytoplankton metabolic activity and cell death. However, fluorescent probes may or may not prove useful for evaluating the physiological state of phytoplankton cells in response to temperature stress. If appropriate protocols are worked out, biological oceanographers will be able to examine and evaluate temperature responses of polar phytoplankton in response to formation and retreat of polar sea ice.