U.S.-Sweden-Estonia Cooperative Research: Immunoglobulin inGene Expression in Transgenic Rainbow Trout

9420481 WARR这一为期三年的奖项将支持美国-瑞典-爱沙尼亚在免疫学方面的合作研究。调查人员是南卡罗来纳医科大学的格雷戈里·沃尔、瑞典乌普萨拉大学的拉尔斯·皮尔斯特罗姆和爱沙尼亚海洋研究所的西耶·蒂姆斯克。他们的研究目的是研究转基因虹鱼中抗体基因的表达。他们提出了以下单细胞研究：(1)斑点叉尾鱼免疫球蛋白增强子在虹鱼中的组织特异性基因表达；(2)虹鱼内源性免疫球蛋白基因转基因表达的影响；以及(3)转基因虹鱼中的功能性嵌合抗体。这位美国研究人员将基因表达和鲶鱼免疫球蛋白基因和增强剂的体外研究专业知识带到了这一合作中。瑞典和爱沙尼亚的研究人员在培育转基因鲑鱼方面的经验以及在体内免疫球蛋白表达的转基因研究方面的专业知识补充了这一点。转基因鱼是在爱沙尼亚海洋研究所培育和维护的。该项目将确定是否可以表达鲶鱼重链基因，如果可以，它是否可以帮助彩虹鲑鱼形成一种功能性抗体。该项目将促进我们对免疫系统和比较免疫学的理解。如果能培育出具有所需特征的转基因鱼，它也有潜在的重要商业应用。***

9420481 Warr This three-year award will support U.S.-Sweden-Estonia cooperative research in immunology. The investigators are Gregory Warr of the Medical University of South Carolina, Lars Pilstrom of Uppsala University in Sweden, and Sirje Timmusk of the Estonian Marine Institute. The objective of their research is the study of antibody gene expression in transgenic rainbow trout. They propose single cell studies of the following: (1) channel catfish immunoglobulin enhancer in tissue-specific gene expression in rainbow trout; (2) impact of transgenic expression of the endogenous immunoglobulin genes of rainbow trout; and (3) functional chimeric antibodies in transgenic rainbow trout. The U.S. investigator brings to this collaboration expertise with in vitro studies of gene expression and catfish immunoglobulin genes and enhancers. This is complemented by the Swedish and Estonian investigators' experience in growing transgenic trout and expertise with in vivo transgenic studies of immunoglobulin expression. The transgenic fish are grown and maintained at the Estonian Marine Institute. The project will determine whether the catfish heavy chain gene can be expressed and if so can it assist with the formation of a functional antibody in rainbow trout. The project will advance our understanding of the immune system and comparative immunology. It also has potentially important commercial applications if transgenic fish with desired traits can be raised. ***